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Population clustering by principle component analysis. The heat maps display the mean of each principle component (rows) for each study population (columns). For example, the mean of the first principle component (PC1) for all volunteers in Peru (PE) is summarized in a single value on the heat map. PCA was performed on (A) immunoglobulin A (IgA) responses and (B) immunoglobulin G (IgG) responses for all in vitro transcription and translation (IVTT) proteins represented on the multipathogen protein microarray (purified protein PCA results are shown in the <xref ref-type= Supplemental Information ). Only the first 10 PCs are shown, capturing approximately 14% and 25% of the variation in the IgA and IgG data, respectively (a heat map of all PCs are shown in Supplemental Information ). Populations and PCs were ordered by hierarchical clustering. The color keys show the scale of gray/yellow lower PC values to red higher PC values. Row labels include percent variation captured by each PC in brackets and P-values from ANOVA of the PC means among study populations. Abbreviations: ETR, rural Ethiopia; ETU, urban Ethiopia; GBR, rural The Gambia; GBU, urban The Gambia; GN, Ghana; KE, Kenya; PE, Peru; SP, Spain; SW, Sweden; USC, U.S.-California; USW, U.S.-Washington; PC#, principal component. " width="100%" height="100%">

Journal: Frontiers in Immunology

Article Title: Multipathogen Analysis of IgA and IgG Antigen Specificity for Selected Pathogens in Milk Produced by Women From Diverse Geographical Regions: The INSPIRE Study

doi: 10.3389/fimmu.2020.614372

Figure Lengend Snippet: Population clustering by principle component analysis. The heat maps display the mean of each principle component (rows) for each study population (columns). For example, the mean of the first principle component (PC1) for all volunteers in Peru (PE) is summarized in a single value on the heat map. PCA was performed on (A) immunoglobulin A (IgA) responses and (B) immunoglobulin G (IgG) responses for all in vitro transcription and translation (IVTT) proteins represented on the multipathogen protein microarray (purified protein PCA results are shown in the Supplemental Information ). Only the first 10 PCs are shown, capturing approximately 14% and 25% of the variation in the IgA and IgG data, respectively (a heat map of all PCs are shown in Supplemental Information ). Populations and PCs were ordered by hierarchical clustering. The color keys show the scale of gray/yellow lower PC values to red higher PC values. Row labels include percent variation captured by each PC in brackets and P-values from ANOVA of the PC means among study populations. Abbreviations: ETR, rural Ethiopia; ETU, urban Ethiopia; GBR, rural The Gambia; GBU, urban The Gambia; GN, Ghana; KE, Kenya; PE, Peru; SP, Spain; SW, Sweden; USC, U.S.-California; USW, U.S.-Washington; PC#, principal component.

Article Snippet: Probed microarrays were scanned using a GenePix 4,300 A high-resolution microarray scanner (Molecular Devices, Sunnyvale, CA), and an image file (.tiff) was saved for each array using GenePix pro 7 software.

Techniques: In Vitro, Microarray, Purification